---
title: Protein analysis in circadian rhythm research
description: Living organisms on earth synchronized activity to a 24 hr light and dark cycle generated by rotation of earth. This biological rhythm is the circadian rhythm.
image: https://blog.mblintl.com/hubfs/CoreFeed1.png
---

**Please Note:** MBL International will be shutting down its operations effective December 31, 2024. Distribution of MBL products in the United States will be transferred to Cosmo Bio US on January 1st while European Distributors will remain unchanged. For any US inquiries regarding orders or support during this transition, reach out to Cosmo Bio: [https://www.cosmobiousa.com/](https://www.cosmobiousa.com/). For Non-US inquiries reach out to MBL in Japan: [https://www.mblbio.com/](https://www.mblbio.com/).

[Skip to the main content.](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#main-content)

- [Distributors](https://mblintl.com/about-mbli/distributors)
- [Contact Us](https://mblintl.com/about-mbli/contact-us)
- [Ordering Information](https://mblintl.com/ordering-information)
- [Login](https://products.mblintl.com/my-account/)
- [View Cart](https://products.mblintl.com/cart/)
- [**QUICK ORDER**](https://products.mblintl.com/my-account/orders/)

[![MLBI](https://blog.mblintl.com/hubfs/MLBI.svg "MLBI")](https://www.mblintl.com)

[![MLBI](https://blog.mblintl.com/hubfs/MLBI.svg "MLBI")](https://www.mblintl.com)

Search

*Search*

[Advanced MHC Tetramers Search](https://products.mblintl.com/advanced-mhc-tetramers-and-antibodies-search/)

Toggle Menu

- Products 
    - Product Type 
          - [Antibodies](https://products.mblintl.com/product-category/antibody/)
          - [Cell Culture](https://products.mblintl.com/product-category/cell-culture/)
          - [Kits](https://products.mblintl.com/product-category/kit/)
          - [MHC Monomers](https://products.mblintl.com/product-category/monomer/)
          - [MHC Tetramers](https://products.mblintl.com/product-category/tetramer/)
          - [Peptides](https://products.mblintl.com/product-category/peptide/)
          - [Purification/Separation](https://products.mblintl.com/product-category/purification/)
          - [Reagents](https://products.mblintl.com/product-category/reagent/)
          - [Recombinant Proteins](https://products.mblintl.com/product-category/recombinant-protein/)
          - [Vectors](https://products.mblintl.com/product-category/vector/)
          - [Western Reagents](https://products.mblintl.com/product-category/western-reagent/)
    - Research Area 
          - [Apoptosis](https://products.mblintl.com/research-area/apoptosis/)
          - [Fluorescent Proteins](https://products.mblintl.com/research-area/fluorescent-proteins/)
          - [Autophagy](https://products.mblintl.com/research-area/autophagy/)
          - [Immunology](https://products.mblintl.com/research-area/immunology/)
          - [Cancer](https://products.mblintl.com/research-area/cancer/)
          - [Metabolism](https://products.mblintl.com/research-area/metabolism/)
          - [Drug Discovery](https://products.mblintl.com/research-area/drug-discovery/)
          - [Neuroscience](https://products.mblintl.com/research-area/neuroscience/)
          - [Epigenetics](https://products.mblintl.com/research-area/epigenetics/)
          - [Organoids](https://products.mblintl.com/research-area/organoid/)
          - [Epitope Tag](https://products.mblintl.com/research-area/epitope-tag/)
          - [Purification/Separation](https://products.mblintl.com/research-area/purification-separation/)
          - [Exosomes](https://products.mblintl.com/research-area/exosomes/)
          - [Smart-IP](https://products.mblintl.com/research-area/smart-ip/)
          - [Flow Cytometry](https://products.mblintl.com/research-area/flow-cytometry/)
- MHC Tetramers & Monomers 
    - MHC Tetramers 
          - [MHC Class I](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-i)
          - [MHC Class II](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-ii)
          - [TCR Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/tcr-tetramers)
          - [Non-Classical MHC Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers)
          - [HLA-E & Qa-1b](https://mblintl.com/hla-e-qa-1b-2)
          - [CD1d Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers/cd1d)
    - MHC Monomers 
          - [MHC Monomers](https://products.mblintl.com/product-category/monomer/)
          - [Tetramer Citations](https://blog.mblintl.com/resources/mhc-tetramers/tetramer-citations)
          - [Peptides](https://products.mblintl.com/product-category/peptide/)
          - [MHC Alleles list](https://mblintl.com/mhc-alelles-list)
          - [Custom MHC Tetramers and Monomers Order Form](https://mblintl.com/custom-mhc-tetramers-and-monomers-order-form-2)
          - [Custom Peptide Order Form](https://mblintl.com/custom-peptide-order-form)
    - Custom Tetramer Kits 
          - [QuickSwitch™ Custom Tetramer Kits](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/)
          - [QuickSwitch™ Peptide Screening Kit](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/quickswitch-peptide-screening-kit/)
          - [SARS-CoV-2 MHC-Peptide Screening Kit Bundles](https://mblintl.com/sars-cov-2-mhc-peptide-screening-kit-bundles-1)
- Research 
    - MHC Tetramers 
          - [Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/)
          - [Monomers](https://blog.mblintl.com/resources/learning-center/research-area/monomers/)
          - [Class I](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-i/)
          - [Class II](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-ii/)
          - [Non-Classical Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers/)
          - [TCR Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/tcr-tetramers/)
          - [CD1d Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers/cd1d/)
          - [QuickSwitch™ Custom Tetramer Kit](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/)
          - [QuickSwitch™ Peptide Screening Kit](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/quickswitch-peptide-screening-kit/)
          - [SARS-CoV-2 Screening Bundles](https://mblintl.com/sars-cov-2-mhc-peptide-screening-kit-bundles-1)
          - [Immune Monitoring FAQ](https://blog.mblintl.com/resources/product-information/faqs-immune-monitoring/)
          - [QuickSwitch™ FAQ](https://mblintl.com/resources/product-information/faqs-quickswitch/)
    - Research Area 
          - [Apoptosis](https://blog.mblintl.com/resources/learning-center/research-area/apoptosis/)
          - [Immuno-oncology](https://blog.mblintl.com/resources/learning-center/research-area/immuno-oncology)
          - [Autophagy](https://blog.mblintl.com/resources/product-information/autophagy/)
          - [*In vivo* Isotype Control Antibodies](https://blog.mblintl.com/resources/learning-center/research-area/in-vivo-grade-isotype-controls/)
          - [Cancer](https://blog.mblintl.com/resources/learning-center/research-area/cancer/)
          - [Knockout Validated Antibodies](https://resources.mblintl.com/learning-center/research-area/knockout-antibodies)
          - [Cancer Neoantigens](https://mblintl.com/research/research-area/cancer-neoantigens)
          - [LC3](https://blog.mblintl.com/resources/product-information/autophagy/lc3-atg8/)
          - [Cell Cycle Indicator](https://blog.mblintl.com/resources/learning-center/research-area/fluorescent-proteins/cell-cycle-indicator/)
          - [Metabolism](https://mblintl.com/research/research-area/metabolism)
          - [Drug Discovery](https://blog.mblintl.com/resources/learning-center/technologies/drug-discovery)
          - [Mitophagy Detection](https://blog.mblintl.com/resources/learning-center/research-area/fluorescent-proteins/mitophagy-detecton/)
          - [Epigenetics](https://mblintl.com/resources/learning-center/research-area/epigenetics)
          - [Neuroscience](https://blog.mblintl.com/resources/learning-center/research-area/neuroscience-reagents)
          - [Exosomes](https://blog.mblintl.com/resources/learning-center/research-area/exosomes/)
          - [Organoids](https://blog.mblintl.com/resources/learning-center/research-area/organoid_culture/)
          - [Fluorescent Proteins/Vectors](https://blog.mblintl.com/resources/learning-center/research-area/fluorescent-proteins/)
          - [PCSK9](https://resources.mblintl.com/research/research-area/pcsk9)
          - [IL 18](https://blog.mblintl.com/resources/learning-center/research-area/immunology/il-18)
          - [Purification/Separation](https://blog.mblintl.com/resources/learning-center/research-area/purification-separation)
          - [Immunology](https://blog.mblintl.com/resources/learning-center/research-area/immunology/)
          - [Western Blot Reagents](https://blog.mblintl.com/resources/scientific-resources/fundamentals-for-planning-research/the-principle-and-method-of-western-blotting-wb/)
- Scientific Resources 
    -   
          - [MHC Tetramer Staining Guide](https://mblintl.com/tetramer-staining-guide-new-2021)
          - [Brochures](https://blog.mblintl.com/resources/product-information/brochures/)
          - [Scientific Reports](https://resources.mblintl.com/scientific-resources/scientific-reports)
          - [Webinars](https://blog.mblintl.com/resources/scientific-resources/webinars/)
    -   
          - [Scientific Posters](https://blog.mblintl.com/resources/product-information/scientific-posters/)
          - [Pathway Posters](https://blog.mblintl.com/resources/product-information/pathway-posters/)
          - [Blogs](https://blog.mblintl.com/)
          - [Videos](https://blog.mblintl.com/resources/product-information/videos/)
    -   
          - [Tetramer Citations](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/tetramer-citations/)
          - [MBLI Product Publications](https://blog.mblintl.com/resources/product-information/citations-publications)
          - [Fundamental Planning for Research](https://blog.mblintl.com/resources/scientific-resources/fundamentals-for-planning-research/)
          - [FAQs](https://blog.mblintl.com/resources/scientific-resources/faqs)
- About 
    - [Distributors](https://mblintl.com/about-mbli/distributors)
    - [About Us](http://mblintl.com/about-mbli)
    - [Contact Us](https://mblintl.com/about-mbli/contact-us)
    - [Support](https://mblintl.com/about-mbli/overview/)
    - [Careers](https://mblintl.com/about-mbli/careers)

Toggle Menu

Search

*Search*

- Products
  
  *Toggle Menu* 
  
    - Product Type
      
      *Toggle Menu* 
      
          - [Antibodies](https://products.mblintl.com/product-category/antibody/)
          - [Cell Culture](https://products.mblintl.com/product-category/cell-culture/)
          - [Kits](https://products.mblintl.com/product-category/kit/)
          - [MHC Monomers](https://products.mblintl.com/product-category/monomer/)
          - [MHC Tetramers](https://products.mblintl.com/product-category/tetramer/)
          - [Peptides](https://products.mblintl.com/product-category/peptide/)
          - [Purification/Separation](https://products.mblintl.com/product-category/purification/)
          - [Reagents](https://products.mblintl.com/product-category/reagent/)
          - [Recombinant Proteins](https://products.mblintl.com/product-category/recombinant-protein/)
          - [Vectors](https://products.mblintl.com/product-category/vector/)
          - [Western Reagents](https://products.mblintl.com/product-category/western-reagent/)
    - Research Area
      
      *Toggle Menu* 
      
          - [Apoptosis](https://products.mblintl.com/research-area/apoptosis/)
          - [Fluorescent Proteins](https://products.mblintl.com/research-area/fluorescent-proteins/)
          - [Autophagy](https://products.mblintl.com/research-area/autophagy/)
          - [Immunology](https://products.mblintl.com/research-area/immunology/)
          - [Cancer](https://products.mblintl.com/research-area/cancer/)
          - [Metabolism](https://products.mblintl.com/research-area/metabolism/)
          - [Drug Discovery](https://products.mblintl.com/research-area/drug-discovery/)
          - [Neuroscience](https://products.mblintl.com/research-area/neuroscience/)
          - [Epigenetics](https://products.mblintl.com/research-area/epigenetics/)
          - [Organoids](https://products.mblintl.com/research-area/organoid/)
          - [Epitope Tag](https://products.mblintl.com/research-area/epitope-tag/)
          - [Purification/Separation](https://products.mblintl.com/research-area/purification-separation/)
          - [Exosomes](https://products.mblintl.com/research-area/exosomes/)
          - [Smart-IP](https://products.mblintl.com/research-area/smart-ip/)
          - [Flow Cytometry](https://products.mblintl.com/research-area/flow-cytometry/)
- MHC Tetramers & Monomers
  
  *Toggle Menu* 
  
    - MHC Tetramers
      
      *Toggle Menu* 
      
          - [MHC Class I](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-i)
          - [MHC Class II](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-ii)
          - [TCR Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/tcr-tetramers)
          - [Non-Classical MHC Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers)
          - [HLA-E & Qa-1b](https://mblintl.com/hla-e-qa-1b-2)
          - [CD1d Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers/cd1d)
    - MHC Monomers
      
      *Toggle Menu* 
      
          - [MHC Monomers](https://products.mblintl.com/product-category/monomer/)
          - [Tetramer Citations](https://blog.mblintl.com/resources/mhc-tetramers/tetramer-citations)
          - [Peptides](https://products.mblintl.com/product-category/peptide/)
          - [MHC Alleles list](https://mblintl.com/mhc-alelles-list)
          - [Custom MHC Tetramers and Monomers Order Form](https://mblintl.com/custom-mhc-tetramers-and-monomers-order-form-2)
          - [Custom Peptide Order Form](https://mblintl.com/custom-peptide-order-form)
    - Custom Tetramer Kits
      
      *Toggle Menu* 
      
          - [QuickSwitch™ Custom Tetramer Kits](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/)
          - [QuickSwitch™ Peptide Screening Kit](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/quickswitch-peptide-screening-kit/)
          - [SARS-CoV-2 MHC-Peptide Screening Kit Bundles](https://mblintl.com/sars-cov-2-mhc-peptide-screening-kit-bundles-1)
- Research
  
  *Toggle Menu* 
  
    - MHC Tetramers
      
      *Toggle Menu* 
      
          - [Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/)
          - [Monomers](https://blog.mblintl.com/resources/learning-center/research-area/monomers/)
          - [Class I](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-i/)
          - [Class II](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/mhc-class-ii/)
          - [Non-Classical Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers/)
          - [TCR Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/tcr-tetramers/)
          - [CD1d Tetramers](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/non-classical-mhc-tetramers/cd1d/)
          - [QuickSwitch™ Custom Tetramer Kit](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/)
          - [QuickSwitch™ Peptide Screening Kit](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/quickswitch-custom-tetramer-kits/quickswitch-peptide-screening-kit/)
          - [SARS-CoV-2 Screening Bundles](https://mblintl.com/sars-cov-2-mhc-peptide-screening-kit-bundles-1)
          - [Immune Monitoring FAQ](https://blog.mblintl.com/resources/product-information/faqs-immune-monitoring/)
          - [QuickSwitch™ FAQ](https://mblintl.com/resources/product-information/faqs-quickswitch/)
    - Research Area
      
      *Toggle Menu* 
      
          - [Apoptosis](https://blog.mblintl.com/resources/learning-center/research-area/apoptosis/)
          - [Immuno-oncology](https://blog.mblintl.com/resources/learning-center/research-area/immuno-oncology)
          - [Autophagy](https://blog.mblintl.com/resources/product-information/autophagy/)
          - [*In vivo* Isotype Control Antibodies](https://blog.mblintl.com/resources/learning-center/research-area/in-vivo-grade-isotype-controls/)
          - [Cancer](https://blog.mblintl.com/resources/learning-center/research-area/cancer/)
          - [Knockout Validated Antibodies](https://resources.mblintl.com/learning-center/research-area/knockout-antibodies)
          - [Cancer Neoantigens](https://mblintl.com/research/research-area/cancer-neoantigens)
          - [LC3](https://blog.mblintl.com/resources/product-information/autophagy/lc3-atg8/)
          - [Cell Cycle Indicator](https://blog.mblintl.com/resources/learning-center/research-area/fluorescent-proteins/cell-cycle-indicator/)
          - [Metabolism](https://mblintl.com/research/research-area/metabolism)
          - [Drug Discovery](https://blog.mblintl.com/resources/learning-center/technologies/drug-discovery)
          - [Mitophagy Detection](https://blog.mblintl.com/resources/learning-center/research-area/fluorescent-proteins/mitophagy-detecton/)
          - [Epigenetics](https://mblintl.com/resources/learning-center/research-area/epigenetics)
          - [Neuroscience](https://blog.mblintl.com/resources/learning-center/research-area/neuroscience-reagents)
          - [Exosomes](https://blog.mblintl.com/resources/learning-center/research-area/exosomes/)
          - [Organoids](https://blog.mblintl.com/resources/learning-center/research-area/organoid_culture/)
          - [Fluorescent Proteins/Vectors](https://blog.mblintl.com/resources/learning-center/research-area/fluorescent-proteins/)
          - [PCSK9](https://resources.mblintl.com/research/research-area/pcsk9)
          - [IL 18](https://blog.mblintl.com/resources/learning-center/research-area/immunology/il-18)
          - [Purification/Separation](https://blog.mblintl.com/resources/learning-center/research-area/purification-separation)
          - [Immunology](https://blog.mblintl.com/resources/learning-center/research-area/immunology/)
          - [Western Blot Reagents](https://blog.mblintl.com/resources/scientific-resources/fundamentals-for-planning-research/the-principle-and-method-of-western-blotting-wb/)
- Scientific Resources
  
  *Toggle Menu* 
  
    -  
      
      *Toggle Menu* 
      
          - [MHC Tetramer Staining Guide](https://mblintl.com/tetramer-staining-guide-new-2021)
          - [Brochures](https://blog.mblintl.com/resources/product-information/brochures/)
          - [Scientific Reports](https://resources.mblintl.com/scientific-resources/scientific-reports)
          - [Webinars](https://blog.mblintl.com/resources/scientific-resources/webinars/)
    -  
      
      *Toggle Menu* 
      
          - [Scientific Posters](https://blog.mblintl.com/resources/product-information/scientific-posters/)
          - [Pathway Posters](https://blog.mblintl.com/resources/product-information/pathway-posters/)
          - [Blogs](https://blog.mblintl.com/)
          - [Videos](https://blog.mblintl.com/resources/product-information/videos/)
    -  
      
      *Toggle Menu* 
      
          - [Tetramer Citations](https://blog.mblintl.com/resources/learning-center/mhc-tetramers/tetramer-citations/)
          - [MBLI Product Publications](https://blog.mblintl.com/resources/product-information/citations-publications)
          - [Fundamental Planning for Research](https://blog.mblintl.com/resources/scientific-resources/fundamentals-for-planning-research/)
          - [FAQs](https://blog.mblintl.com/resources/scientific-resources/faqs)
- About
  
  *Toggle Menu* 
  
    - [Distributors](https://mblintl.com/about-mbli/distributors)
    - [About Us](http://mblintl.com/about-mbli)
    - [Contact Us](https://mblintl.com/about-mbli/contact-us)
    - [Support](https://mblintl.com/about-mbli/overview/)
    - [Careers](https://mblintl.com/about-mbli/careers)

- [Distributors](https://mblintl.com/about-mbli/distributors)
- [Contact Us](https://mblintl.com/about-mbli/contact-us)
- [Ordering Information](https://mblintl.com/ordering-information)
- [Login](https://products.mblintl.com/my-account/)
- [View Cart](https://products.mblintl.com/cart/)
- [**QUICK ORDER**](https://products.mblintl.com/my-account/orders/)

[*Facebook*](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#0) [*Instagram*](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#0) [*LinkedIn*](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#0) [*Twitter*](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#0) [*Youtube*](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#0) [*Medium*](https://blog.mblintl.com/protein-analysis-in-circadian-rhythm-research#0)

- [Home](https://blog.mblintl.com/)
- [Blogs](https://blog.mblintl.com)
- Protein analysis in circadian rhythm research

# Protein analysis in circadian rhythm research

### Topics

- Select Topic - 3D Cell Culture Advancements in Metabolism Research Allergy Antibodies Antibody Antibody Reagents Antibody-Peptide Conjugates Apoptosis Assay and Kits Autoimmune Disease Autophagy BRIC Benefits of Tetramer Assays Biomarker Cancer Cell Biology Cell Culture Reagents Cell Stabilization Custom Peptide Synthesis Cytokine Dextramer Staining Drug Discovery ELISA Epigenetics Epitope Tag Exosome Exosomes FCM Fc blocker Flow Cytometry Flow Cytometry Antibodies Fluoppi Fluorescent Proteins Future of Tetramer Technology Gastric Inhibitory Peptide Gene Regulation Gene Therapy Green Fluorescent Protein IL-18 Immune Response Immunology Inflammation Isotype Control Kits and Assays LC3 Liquid Biopsy MHC MHC Alleles MHC Class II Tetramers MHC Peptides Contribute MHC Tetramer MHC Tetramer Protocol MHC tetramer advancements Metabolism Mitophagy NCP Neuroscience Neuroscience Reagents Organoid PCSK9 PCSK9 ELISA Kit Peptide Peptide Exchange Peptide Synthesis Methods Peptide Synthesis Service Peptide-Infused Skin Care Products Preservation Purify exosome Quality Control QuickSwitch Quickswitch™ Custom Tetramer Kits RBP RIP Chip RNA Recombinant Protein Production Recombinant Proteins Regulation RiboTrap S100 Proteins SARS-CoV-2 T-Cell Detection TCR Tetramers Tetramer Tetramer Analysis Tetramer Assay Tetramer Assay Protocol Tetramer Staining TransFix Vectors Western Reagents atg8 covid-19 gfp keima red kinases mitochondria cell death p62 pathways phosphorylation pro IL-18 reagents rfp t cell tuberculosis ultracentrifugation vaccine

### Top Posts

### Subscribe to our Blogs

Published by [Tomohiro Nakajo](https://blog.mblintl.com/author/tomohiro-nakajo) on Sep 27, 2017 12:00:00 PM

![](https://blog.mblintl.com/hubfs/CoreFeed1.png)

Living organisms on the earth synchronize their activity to a 24 hour light and dark cycle generated by the rotation of the earth. This biological rhythm is called the circadian rhythm.

Recently the molecular mechanism for the oscillation of the circadian rhythm has been elucidated and the approximately 24-hour-rhythm was found to be generated by a transcription-translation feedback-loop of clock genes expressed by almost all cells. In particular, BMAL1, CLOCK, PERs and CRYs play key roles in oscillation of the circadian rhythm and rhythmically regulate the expression of downstream genes (hereinafter referred to as clock-controlled genes \[CCGs\]).

So far, the expression analysis of CCGs is mainly performed by mRNA quantification, like qRT-PCR and in situ hybridization. However, it is necessary to analyze protein expression level, post-translational modifications like phosphorylation, and ubiquitination for understanding the molecular mechanism of circadian rhythm. Actually, phosphorylation and ubiquitination of PER and ubiquitination of CRY are well known.

Why are these protein expression analyses not well done? The answer is very simple: because there weren't any good antibodies in this market, so we developed antibodies against CCGs.

![CoreFeed1.png](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed1.png?width=313&name=CoreFeed1.png)

At first we developed antibodies against PER1/2, CRY1/2, BMAL1 and CLOCK, playing core feedback loop in circadian rhythm. BMAL1 and CLOCK form a heterodimer, which binds to the regulatory region (E-box) in *per1/2* and *cry1/2* to positively regulate transcription. PER and CRY proteins then form a complex, migrate to the nucleus, and negatively regulate the function of the BMAL1/CLOCK complex. CCGs with E-box elements are expressed and are regulated by the BMAL1/CLOCK complex, and various genes are expressed according to circadian rhythm.

We successfully developed antibodies against all of the core feedback loop members. Anti-PER1, PER2, BMAL1 and CLOCK can be used in Immunohistochemistry (IHC), and anti-BMAL1 and CLOCK can be used for Chromatin Immunoprecipitation (ChIP) assays as well. Please be careful to choose secondary antibodies because some of the products are derived from guinea pig!

**Anti-PER2 (Mouse) pAb ([Code# PM083](https://products.mblintl.com/products/pm083)) –Rabbit Ig (Aff.)**

![CoreFeed11.jpg](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed11.jpg?width=618&height=282&name=CoreFeed11.jpg)

**Anti-PER1(Mouse) pAb ([Code# PM091](https://products.mblintl.com/products/pm091)) - Guinea Pig Ig (Aff.)**

![CoreFeed5.jpg](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed5.jpg?width=622&name=CoreFeed5.jpg)

**Anti-CRY1(Mouse) pAb ([Code# PM081](https://products.mblintl.com/products/pm081)) - Guinea Pig Ig (Aff.)**

![CoreFeed8.jpg](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed8.jpg?width=618&height=227&name=CoreFeed8.jpg)

**Anti-CRY2 (Mouse) pAb ([Code# PM082](https://products.mblintl.com/products/pm082)) - Guinea Pig Ig (Aff.)**

![CoreFeed7.jpg](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed7.jpg?width=618&height=229&name=CoreFeed7.jpg)

**Anti-BMAL1 mAb ([Code# D361-3](https://products.mblintl.com/products/d361-3))**

![CoreFeed9.jpg](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed9.jpg?width=618&height=254&name=CoreFeed9.jpg)

Anti-CLOCK (Mouse) mAb ([Code# D349-3](https://products.mblintl.com/products/d349-3))

![CoreFeed10-1.jpg](https://blog.mblintl.com/hs-fs/hubfs/CoreFeed10-1.jpg?width=621&name=CoreFeed10-1.jpg)

**Other antibodies related to circadian rhythm are also available.**

| **Code #** | **Product Name** | **Clone** | **Isotype** | **Application** |
| --- | --- | --- | --- | --- |
| [PM091](https://products.mblintl.com/products/pm091) | Anti-Per1 (Mouse) pAb | Polyclonal | Guinea Pig Ig (aff.) | WB/IP |
| [PM083](https://products.mblintl.com/products/pm083) | Anti-Per2 (Mouse) pAb | Polyclonal | Rabbit Ig. (aff.) | WB/IP/IH |
| [PM096](https://products.mblintl.com/products/pm096) | Anti-Per2 (Human) pAb | Polyclonal | Guinea Pig Ig (aff.) | WB/IP |
| [PM081](https://products.mblintl.com/products/pm081) | Anti-Cry1 (Mouse) pAb | Polyclonal | Guinea Pig IgG | WB/IP |
| [PM082](https://products.mblintl.com/products/pm082) | Anti-Cry2 (Mouse) pAb | Polyclonal | Guinea Pig IgG | WB/IP |
| [D361-3](https://products.mblintl.com/products/d361-3) | Anti-BMAL mAb | 2F11 | Mouse IgG1 κ | WB/IH |
| [D335-3](https://products.mblintl.com/products/d335-3) | Anti-BMAL (Mouse) mAb | B1BH2 | Mouse IgG1 κ | WB/ChIP\* |
| [D349-3](https://products.mblintl.com/products/d349-3) | Anti-CLOCK (Mouse) mAb | CLSP4 | Mouse IgG1 κ | WB/IP/IH/ChIP\* |
| [D333-3](https://products.mblintl.com/products/d333-3) | Anti-CLOCK (Mouse) mAb  | CLSP3 | Mouse IgG1 κ | WB/IP\*\*/ChIP\* |
| [D334-3](https://products.mblintl.com/products/d334-3) | Anti-CLOCK (Mouse) mAb | CLNT1 | Mouse IgG1 κ | WB/IP\* |
| [PM087](https://products.mblintl.com/products/pm087) | Anti-Chrono (Mouse) mAb | Polyclonal | Rabbit Ig (aff.) | WB |
| [PM079](https://products.mblintl.com/products/pm079) | Anti-DBP (Mouse) pAb | Polyclonal | Rabbit Ig (aff.) | WB/IF |
| [PM097](https://products.mblintl.com/products/pm097) | Anti-NFIL3 (E4BP4) pAb | Polyclonal | Rabbit Ig (aff.) | WB |
| [M225-3](https://products.mblintl.com/products/m225-3) | Anti-NFIL3 (E4BP4) chimeric mAb | 42 | Guinea Pig Ig κ (chimeric) | WB/IP/FCM/IH |
| [PM093](https://products.mblintl.com/products/pm093) | Anti-NR1D2 (Rec-erbß) pAb | Polyclonal | Guinea Pig Ig (aff.) | WB/IP |
| [PM092](https://products.mblintl.com/products/pm092) | Anti-NR1D1 (Rev-erbα) pAb | Polyclonal | Rabbit Ig (aff.) | WB/IP |
| [PM075](https://products.mblintl.com/products/pm075) | Anti-GNAT2 (Zebrafish) pAb | Polyclonal | Rabbit Ig (aff.) | WB/IF |

|  \*The use is reported in a research article (Not tested by MBL). Please check the datasheet for detailed information. |
| --- |
|  \*\*The use is reported from the licenser (Under evaluation or not tested by MBL). |
|   |

 

As described above, many CCGs are taking various post-translational modifications, and it becomes clear that these modifications are important to regulate oscillation of the circadian rhythm.

We hope our antibodies help you to elucidate spatiotemporal modification of CCGs, and become 'good antibodies'! 

 

Tags: [Antibodies](https://blog.mblintl.com/topic/antibodies)

---

[Back to Blog](https://blog.mblintl.com)

---

### Comments Or Suggestions

## Related Articles

### Sign up for our latest news and promotions. [![SIGN UP](https://no-cache.hubspot.com/cta/default/424171/d32f72bb-0a07-486f-b8c5-1a2cb67d95fb.png)](https://cta-redirect.hubspot.com/cta/redirect/424171/d32f72bb-0a07-486f-b8c5-1a2cb67d95fb)

##### Research Products

- - [Antibodies](https://products.mblintl.com/product-category/antibody/)
    - [Cell Culture](https://products.mblintl.com/product-category/cell-culture/)
    - [Kits](https://products.mblintl.com/product-category/kit/)
    - [MHC Tetramers](https://products.mblintl.com/product-category/tetramer/)
    - [MHC Monomers](https://products.mblintl.com/product-category/monomer/)
    - [Peptides](https://products.mblintl.com/product-category/peptide/)
- - [Reagents](https://products.mblintl.com/product-category/reagent/)
    - [Recombinant Proteins](https://products.mblintl.com/product-category/recombinant-protein/)
    - [Purification/Separation](https://products.mblintl.com/product-category/purification/)
    - [Vectors](https://products.mblintl.com/product-category/vector/)
    - [Western Reagents](https://products.mblintl.com/product-category/western-reagent/)

##### Contact Us

**MBL International**  
1201 Wiley Rd  
Suite 150  
Schaumburg, IL 60173  
USA

A JSR Life Sciences Company

##### Find Us On

[*Facebook*](https://www.facebook.com/pages/MBL-International-Corporation/313514772031567)[*LinkedIn*](https://www.linkedin.com/company/mbl-international)[*RSS*](https://blog.mblintl.com/)[*Twitter*](https://twitter.com/MBLICorp)

[![jsrls-logo-1](https://blog.mblintl.com/hubfs/Assets/jsrls-logo-1.svg)](https://blog.mblintl.com/)

- [Privacy Policy](https://products.mblintl.com/privacy-policy/)
- [Terms of Use](https://products.mblintl.com/legal/)
- [Sitemap](https://products.mblintl.com/site-map/)
- [Terms and Conditions of Sale](https://products.mblintl.com/terms-conditions/)

Copyright © 2026. All Rights Reserved

*Return to Top*