The Lab Notebook
IL-18 Therapies Targeting Inflammatory Disease
Interleukin-18 (IL-18) is an effective pro-inflammatory cytokine for the host defense against...
Recent Peptide-MHC Exchange Publications Using QuickSwitch™ Technology
MHC-peptide exchange is considered to be tricky by researchers. However, with the right set of...
Crucial Niche Factors for Organoid Cultures
Organoids are 3D structures made up of cells that replicate the structure and function of tissues...
The various and complex components of the innate immune response
Innate Immunity is a nonspecific host defense mechanism against foreign antigens. It is the first...
2022 RUO Publication Newsletter
MBLI is proud to continuously provide high quality research reagents to the biological community,...
The Impact of In Vivo Isotype Controls on Therapeutic Drug Development
The significance of antibody based in vivo studies For both research and therapeutic purposes,...
Stop Crying in the Lab: How Cry Antibodies Can Help Your Research
Figure: Schematic representation of feedback loop involving CLOCK-BMAL1 complex and CRY and PER...
Why should you use Isotype Control Antibodies
Good experimental design is key to accurate and precise drug development. Isotype control...
Human MHC Class II QuickSwitch™ Platform is used to Identify SARS-CoV-2 Virus Derived Peptides
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have...
How to Identify SARS-CoV-2 Virus Derived Peptides
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have...
Find Human MHC Class I Dominant Peptides
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have...
Comparing Theoretical Peptide-MHC Binding Affinity to Real Values produced by the QuickSwitch™ Platform
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have
How to screen SARS-CoV-2 peptides to facilitate T cell research
Whenever our immune system is faced with a challenge, be it a multicellular parasite, a bacteria, a...
MuLV gp70 (AH1) Tetramer - a great tool for observing antigen specific immune response in cancer
MuLV gp 70, also designated as AH1 (SPSYVYHQF), is an endogenous viral envelope glycoprotein...
In vivo NIRF whole-body imaging through MHC tetramers
The use of MHC tetramers for the detection of antigen specific T cells has been a well-established...
Why Smart-IP is the smart way to IP
You have been given the mission to start a new project. A tiny tube is put in your hands and you’re...
Screening for COVID-19 T-cell peptides and immune monitoring with MHC tetramers in a single assay
COVID-19 infected cells can be recognized by T-cells only after SARS-CoV-2 peptides are processed...
Cancer Immunotherapy - Growing focus on CD4+ T lymphocytes and MHC Class II Neoantigens
It is now well accepted that CD8+ T cells play a central role in mediating anti-tumor immunity by...
Is your drug candidate able to reverse the T Cell Exhaustion-like state in our in vitro functional screening assay?
The goal of cancer immune checkpoint therapies is to cure tumor-specific T cells from dysfunction...
Blocking Fc receptors for flow cytometry
While, in theory, using antibodies against Fc Receptors (FcRs) is the best way to eliminate...
New tetramer for vaccine research targeting Tuberculosis and Ag85B specific T-cells
Tuberculosis (TB) is caused by the Mycobacterium tuberculosis and usually targets the lungs. This...
Why protein phosphorylation is crucial in disease research
Protein phosphorylation is quintessential for specific pathways to function. This function can be...
QuickSwitch, a tool for adoptive T cell transfer
Our understanding of how the immune system responds to cancer has increased by leaps and bounds in...
Stabilizing cellular antigens the easy way using Transfix
Many researchers and clinicians have issues with keeping their whole blood or CSF samples viable...
Is Wnt3a an essential component in organoid culture?
Organoids are self-organized three-dimensional tissue cultures that are derived from mammalian...
3D cell imaging made easier
There is an increase in demand for scientists to show cell migration and proliferation on a surface...
LC3: The gold standard for autophagy research
LC3, a mammalian homologue of yeast ATG8, is a soluble protein that is distributed ubiquitously in...
Gating strategy for tetramer analysis to eliminate non-specific event
While MHC tetramer staining of antigen specific T cells for flow cytometry is considered highly...
Shed a little light in your cells using anti-GFP
In 2008, the Nobel Prize in Chemistry was awarded to Dr. Roger Y. Tsien, Dr. Osamu Shimomura, and...
Which tetramers can be used for my mouse samples?
The thymus shapes a host's T-cell repertoire so they do not respond to "self" antigens, yet are...
Learn why IL-18 is an exciting biomarker for various diseases
As described in previous blog posts, our IL-18 related products are used widely among researchers...
Improve your neutralization using monoclonal IL-18 antibodies
Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor...
Tips for successful results using our extremely popular IL-18 ELISA kits
Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor...
How do I even get started? Lessons for setting up your experiments
Biology can be daunting. Experiments can be daunting. Setting up your own biological experiments...
How to detect functional PCSK9 quickly and easily
PCSK9 remains an important target in cholesterol research. One of the hurdles in this research...
Can NanoCulture Plate (NCP) be a better 3D cell culture platform?
Cell-based assays are the first step for drug screening applications and have been performed in...
Quick! Switch out your peptide!
The immunotherapy field is making great progress in aiding many disease treatments. The hope is...
Antibodies for fluorescent proteins: Will it cross react with my variant?
Green fluorescent protein (GFP) was first isolated by Dr. Osamu Shimomura in 1961. Since then,...
S100 Proteins: Study their Hundreds of Functions with MBL ELISA Kits
Have you heard of the S100 protein family? S100 refers to proteins that are 100% soluble in...
Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.
Mechanisms of Autophagy The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold...
Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform
Immuno-oncology is a growing field in which different strategies such as immune modulators,...
The problem with Ultracentrifugation for exosome isolation
Ultracentrifugation is regarded as the gold standard method for exosome isolation. The...
Protein analysis in circadian rhythm research
Living organisms on the earth synchronize their activity to a 24 hour light and dark cycle...
How to detect functional PCSK9 quickly and easily
PCSK9 remains an important target in cholesterol research. One of the hurdles in this research...
Why NCP 3D cell culture method helps your experiments become more real
3D cell culture innovation ensures that time spent at the bench will correlate to in vivo...
Solving cellular degradation issues with TransFix
Do your samples break down before you can analyze them? TransFix is an easy and effective method to...
Why pure exosome isolation matters
Exosomes are cell-derived vesicles originating from multi-vesicular bodies and found in biological...
Learn about MBL's QC process for providing high quality antibodies!
The path that an MBL antibody takes to go from development and manufacturing to the benchtop is...
Quick! Switch out your peptide!
The immunotherapy field is making great progress in aiding many disease treatments. The hope is...
4 Cool Science Discoveries in 2016
As 2016 draws to a close, let’s look back on some significant advances made in science.
Why Autophagy research won a Nobel Prize
The process of autophagy was first recognized in the late 1950s and it was thought of as bulk...
What does mitochondria have to do with mitophagy?
What is mitophagy? It is when damaged mitochondria are removed from the cell by autophagy. The...
Don't be fooled. Not all tetramers are created equal!
Have you seen comparisons of multimer technology claiming to be superior to tetramers? Be sure to...
How to choose the right vector for your fluorescent protein needs!
MBL’s CoralHue fluorescent product series is composed of many proteins to make your life...
How to find and read a publication
When you start working in a new lab or working on a new research project, one of the first things...
Who’s that on my mRNA?
You have an RNA of interest aka your favorite RNA. The question is: which RBPs interact with it?...
Don’t rip your hair out- use our RIP certified antibodies!
What are RBPs (RNA binding proteins)? Very briefly, they are proteins that bind to RNA. (You...
Improve your RNA research using our anti-BrdU antibody!
How do you quantify newly synthesized RNA? There are several ways to detect synthesized RNA, but...
Don't FRET- You've got Fluoppi!
Visualizing protein-protein interaction (PPI) is pretty neat. You are able to glimpse into a...Colorimetric? Fluorometric? How to choose the right platform for your ELISA
What happens when you use a black well plate for a fluorometric ELISA assay reading? What happens...
Help! My mouse tetramer stained ALL the CD8+ T cells!
You’re sitting at your flow cytometer, staring at your CD8 x tetramer plot in anticipation of a...
Autophagy Watch...What the Flux is that all about?
What does Autophagy Flux even mean? It’s not in the Urban dictionary. Dear reader, you have come...
Give Your Research 100% With a S100 Antibody
Figure: Schematic representation of S100 proteins in the MAP kinase and NF-kappaB pathways. S100...
Elevate Your Research: New Pathways of Study For Your PCSK9 ELISA Kit
Figure: Schematic representation of PCSK-cIAP1 Binding Assay Kit which can be used to screen...
Elevate Your Cholesterol Research: How a PCSK9 ELISA Kit Can Help You
Ever wonder how cholesterol levels are regulated? One of the major regulators is a molecule...
Why Smart-IP is the smart way to IP
You have been given the mission to start a new project. A tiny tube is put in your hands and you’re...
The Fas and the Furious 2: The Importance of Fas in Cancer
Given the prominent role of Fas in many important cell functions (7 Facts to Catch You Up to...
The Fas and the Furious: 7 Facts to Catch You Up to Speed on Fas and How a Fas Antibody Can Help Your Research
Fas antigen is a receptor protein that has been linked to many diseases such as cancer1 and...
Be positive! 5 ways to confirm your MHC tetramer is binding
In the previous Tetramer Tips blog, I suggested ways for you to be a negative Control Freak. Now...