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The Lab Notebook
ELISA
,
Assay and Kits
,
Cell Biology
,
Immune Response
,
Antibodies
,
Biomarker
,
Inflammation
Integrin αvβ6: An indicator of inflammation and ulcerative colitis
Integrins are heterodimeric cell surface receptors involved in cell-extracellular matrix and...
Read More
Tetramer
,
Immunology
,
Flow Cytometry
,
Immune Response
,
MHC
Correlation Between HLA Typing and Immune Monitoring in Human Disease
The HLA (human leukocyte antigen) system, also known as the human MHC (major histocompatibility...
Read More
Immunology
,
IL-18
,
Kits and Assays
,
Cell Biology
,
Autoimmune Disease
,
cytokine
IL-18 Therapies Targeting Inflammatory Disease
Interleukin-18 (IL-18) is an effective pro-inflammatory cytokine for the host defense against...
Read More
Immunology
,
QuickSwitch
,
Peptide Exchange
,
MHC
Recent Peptide-MHC Exchange Publications Using QuickSwitch™ Technology
MHC-peptide exchange is considered to be tricky by researchers. However, with the right set of...
Read More
3D Cell Culture
,
organoid
Crucial Niche Factors for Organoid Cultures
Organoids are 3D structures made up of cells that replicate the structure and function of tissues...
Read More
Immunology
,
Kits and Assays
,
Immune Response
The various and complex components of the innate immune response
Innate Immunity is a nonspecific host defense mechanism against foreign antigens. It is the first...
Read More
mitophagy
,
Autophagy
,
Apoptosis
,
Epigenetics
,
organoid
2022 RUO Publication Newsletter
MBLI is proud to continuously provide high quality research reagents to the biological community,...
Read More
Antibodies
,
isotype control
The Impact of In Vivo Isotype Controls on Therapeutic Drug Development
The significance of antibody based in vivo studies For both research and therapeutic purposes,...
Read More
Stop Crying in the Lab: How Cry Antibodies Can Help Your Research
Figure: Schematic representation of feedback loop involving CLOCK-BMAL1 complex and CRY and PER...
Read More
Antibodies
,
isotype control
Why should you use Isotype Control Antibodies
Good experimental design is key to accurate and precise drug development. Isotype control...
Read More
Tetramer
,
QuickSwitch
,
SARS-CoV-2
Human MHC Class II QuickSwitch™ Platform is used to Identify SARS-CoV-2 Virus Derived Peptides
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have...
Read More
Tetramer
,
QuickSwitch
,
SARS-CoV-2
How to Identify SARS-CoV-2 Virus Derived Peptides
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have...
Read More
Tetramer
,
QuickSwitch
,
SARS-CoV-2
Find Human MHC Class I Dominant Peptides
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have...
Read More
Tetramer
,
QuickSwitch
,
SARS-CoV-2
Comparing Theoretical Peptide-MHC Binding Affinity to Real Values produced by the QuickSwitch™ Platform
In our previous blog post (How to screen SARS-CoV-2 peptides to facilitate T cell research) we have
Read More
Tetramer
,
QuickSwitch
,
covid-19
,
SARS-CoV-2
How to screen SARS-CoV-2 peptides to facilitate T cell research
Whenever our immune system is faced with a challenge, be it a multicellular parasite, a bacteria, a...
Read More
Tetramer
MuLV gp70 (AH1) Tetramer - a great tool for observing antigen specific immune response in cancer
MuLV gp 70, also designated as AH1 (SPSYVYHQF), is an endogenous viral envelope glycoprotein...
Read More
Tetramer
,
QuickSwitch
In vivo NIRF whole-body imaging through MHC tetramers
The use of MHC tetramers for the detection of antigen specific T cells has been a well-established...
Read More
Epitope Tag
Why Smart-IP is the smart way to IP
You have been given the mission to start a new project. A tiny tube is put in your hands and you’re...
Read More
Tetramer
,
Peptide
,
covid-19
,
SARS-CoV-2
,
vaccine
,
MHC
Screening for COVID-19 T-cell peptides and immune monitoring with MHC tetramers in a single assay
COVID-19 infected cells can be recognized by T-cells only after SARS-CoV-2 peptides are processed...
Read More
Tetramer
,
Cancer
Cancer Immunotherapy - Growing focus on CD4+ T lymphocytes and MHC Class II Neoantigens
It is now well accepted that CD8+ T cells play a central role in mediating anti-tumor immunity by...
Read More
Tetramer
,
Cancer
,
t cell
Is your drug candidate able to reverse the T Cell Exhaustion-like state in our in vitro functional screening assay?
The goal of cancer immune checkpoint therapies is to cure tumor-specific T cells from dysfunction...
Read More
Cell Biology
,
Antibodies
,
Fc blocker
Blocking Fc receptors for flow cytometry
While, in theory, using antibodies against Fc Receptors (FcRs) is the best way to eliminate...
Read More
Tetramer
,
Immunology
,
tuberculosis
New tetramer for vaccine research targeting Tuberculosis and Ag85B specific T-cells
Tuberculosis (TB) is caused by the Mycobacterium tuberculosis and usually targets the lungs. This...
Read More
Antibodies
,
phosphorylation
,
pathways
,
kinases
Why protein phosphorylation is crucial in disease research
Protein phosphorylation is quintessential for specific pathways to function. This function can be...
Read More
Tetramer
,
QuickSwitch
QuickSwitch, a tool for adoptive T cell transfer
Our understanding of how the immune system responds to cancer has increased by leaps and bounds in...
Read More
FCM
,
TransFix
,
Preservation
,
Cell Stabilization
Stabilizing cellular antigens the easy way using Transfix
Many researchers and clinicians have issues with keeping their whole blood or CSF samples viable...
Read More
3D Cell Culture
,
organoid
Is Wnt3a an essential component in organoid culture?
Organoids are self-organized three-dimensional tissue cultures that are derived from mammalian...
Read More
3D Cell Culture
,
NCP
3D cell imaging made easier
There is an increase in demand for scientists to show cell migration and proliferation on a surface...
Read More
Autophagy
,
LC3
,
atg8
LC3: The gold standard for autophagy research
LC3, a mammalian homologue of yeast ATG8, is a soluble protein that is distributed ubiquitously in...
Read More
Tetramer
,
Flow Cytometry
Gating strategy for tetramer analysis to eliminate non-specific event
While MHC tetramer staining of antigen specific T cells for flow cytometry is considered highly...
Read More
Antibodies
,
rfp
,
gfp
Shed a little light in your cells using anti-GFP
In 2008, the Nobel Prize in Chemistry was awarded to Dr. Roger Y. Tsien, Dr. Osamu Shimomura, and...
Read More
Tetramer
Which tetramers can be used for my mouse samples?
The thymus shapes a host's T-cell repertoire so they do not respond to "self" antigens, yet are...
Read More
IL-18
,
ELISA
,
pro IL-18
Learn why IL-18 is an exciting biomarker for various diseases
As described in previous blog posts, our IL-18 related products are used widely among researchers...
Read More
IL-18
,
ELISA
,
pro IL-18
Improve your neutralization using monoclonal IL-18 antibodies
Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor...
Read More
IL-18
,
ELISA
,
pro IL-18
Tips for successful results using our extremely popular IL-18 ELISA kits
Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor...
Read More
How do I even get started? Lessons for setting up your experiments
Biology can be daunting. Experiments can be daunting. Setting up your own biological experiments...
Read More
PCSK9
,
Metabolism
,
PCSK9 ELISA Kit
How to detect functional PCSK9 quickly and easily
PCSK9 remains an important target in cholesterol research. One of the hurdles in this research area...
Read More
3D Cell Culture
,
NCP
Can NanoCulture Plate (NCP) be a better 3D cell culture platform?
Cell-based assays are the first step for drug screening applications and have been performed in...
Read More
Tetramer
,
QuickSwitch
,
Peptide
,
Peptide Exchange
Quick! Switch out your peptide!
The immunotherapy field is making great progress in aiding many disease treatments. The hope is...
Read More
Fluorescent Proteins
,
Antibodies
Antibodies for fluorescent proteins: Will it cross react with my variant?
Green fluorescent protein (GFP) was first isolated by Dr. Osamu Shimomura in 1961. Since then,...
Read More
ELISA
,
S100 Proteins
S100 Proteins: Study their Hundreds of Functions with MBL ELISA Kits
Have you heard of the S100 protein family? S100 refers to proteins that are 100% soluble in...
Read More
Autophagy
,
p62
Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.
Mechanisms of Autophagy The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold...
Read More
Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform
Immuno-oncology is a growing field in which different strategies such as immune modulators,...
Read More
Exosomes
,
ultracentrifugation
The problem with Ultracentrifugation for exosome isolation
Ultracentrifugation is regarded as the gold standard method for exosome isolation. The...
Read More
Antibodies
Protein analysis in circadian rhythm research
Living organisms on the earth synchronize their activity to a 24 hour light and dark cycle...
Read More
PCSK9
,
Metabolism
,
PCSK9 ELISA Kit
How to detect functional PCSK9 quickly and easily
PCSK9 remains an important target in cholesterol research. One of the hurdles in this research area...
Read More
Cell Biology
,
3D Cell Culture
,
NCP
Why NCP 3D cell culture method helps your experiments become more real
3D cell culture innovation ensures that time spent at the bench will correlate to in vivo...
Read More
Cancer
,
Exosomes
,
Liquid Biopsy
,
Exosome
,
Biomarker
,
Purify exosome
Why pure exosome isolation matters
Exosomes are cell-derived vesicles originating from multi-vesicular bodies and found in biological...
Read More
Kits and Assays
,
Quality Control
,
Antibodies
Learn about MBL's QC process for providing high quality antibodies!
The path that an MBL antibody takes to go from development and manufacturing to the benchtop is...
Read More
Tetramer
,
QuickSwitch
,
Peptide
,
Peptide Exchange
Quick! Switch out your peptide!
The immunotherapy field is making great progress in aiding many disease treatments. The hope is...
Read More
4 Cool Science Discoveries in 2016
As 2016 draws to a close, let’s look back on some significant advances made in science.
Read More
Autophagy
Why Autophagy research won a Nobel Prize
The process of autophagy was first recognized in the late 1950s and it was thought of as bulk...
Read More
mitophagy
,
Autophagy
,
mitochondria cell death
,
keima red
What does mitochondria have to do with mitophagy?
What is mitophagy? It is when damaged mitochondria are removed from the cell by autophagy. The...
Read More
Tetramer
Don't be fooled. Not all tetramers are created equal!
Have you seen comparisons of multimer technology claiming to be superior to tetramers? Be sure to...
Read More
Fluoppi
,
Fluorescent Proteins
,
Vectors
How to choose the right vector for your fluorescent protein needs!
MBL’s CoralHue fluorescent product series is composed of many proteins to make your life...
Read More
How to find and read a publication
When you start working in a new lab or working on a new research project, one of the first things...
Read More
RNA
,
Epigenetics
,
Gene Regulation
,
RiboTrap
,
RBP
Who’s that on my mRNA?
You have an RNA of interest aka your favorite RNA. The question is: which RBPs interact with it?...
Read More
RIP Chip
,
RNA
,
Epigenetics
,
Gene Regulation
Don’t rip your hair out- use our RIP certified antibodies!
What are RBPs (RNA binding proteins)? Very briefly, they are proteins that bind to RNA. (You...
Read More
BRIC
,
RNA
,
Epigenetics
,
Regulation
Improve your RNA research using our anti-BrdU antibody!
How do you quantify newly synthesized RNA? There are several ways to detect synthesized RNA, but...
Read More
Fluoppi
,
Fluorescent Proteins
Don't FRET- You've got Fluoppi!
Visualizing protein-protein interaction (PPI) is pretty neat. You are able to glimpse into a...
Read More
Kits and Assays
Colorimetric? Fluorometric? How to choose the right platform for your ELISA
What happens when you use a black well plate for a fluorometric ELISA assay reading? What happens...
Read More
Tetramer
Help! My mouse tetramer stained ALL the CD8+ T cells!
You’re sitting at your flow cytometer, staring at your CD8 x tetramer plot in anticipation of a...
Read More
Autophagy
Autophagy Watch...What the Flux is that all about?
What does Autophagy Flux even mean? It’s not in the Urban dictionary. Dear reader, you have come to...
Read More
Immunology
,
Apoptosis
,
Cancer
Give Your Research 100% With a S100 Antibody
Figure: Schematic representation of S100 proteins in the MAP kinase and NF-kappaB pathways. S100...
Read More
Apoptosis
Elevate Your Research: New Pathways of Study For Your PCSK9 ELISA Kit
Figure: Schematic representation of PCSK-cIAP1 Binding Assay Kit which can be used to screen...
Read More
Metabolism
Elevate your Cholesterol Research Part 2: How Our PCSK9 ELISA Kit Can Help You
Read More
Metabolism
Elevate Your Cholesterol Research: How a PCSK9 ELISA Kit Can Help You
Ever wonder how cholesterol levels are regulated? One of the major regulators is a molecule...
Read More
Epitope Tag
Why Smart-IP is the smart way to IP
You have been given the mission to start a new project. A tiny tube is put in your hands and you’re...
Read More
Apoptosis
,
Cancer
The Fas and the Furious 2: The Importance of Fas in Cancer
Given the prominent role of Fas in many important cell functions (7 Facts to Catch You Up to...
Read More
Apoptosis
The Fas and the Furious: 7 Facts to Catch You Up to Speed on Fas and How a Fas Antibody Can Help Your Research
Fas antigen is a receptor protein that has been linked to many diseases such as cancer1 and...
Read More
Topics
3D Cell Culture
(5)
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(9)
Apoptosis
(5)
Assay and Kits
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atg8
(1)
Autoimmune Disease
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Autophagy
(7)
Biomarker
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BRIC
(1)
Cancer
(5)
Cell Biology
(4)
Cell Stabilization
(1)
covid-19
(2)
cytokine
(1)
ELISA
(5)
Epigenetics
(4)
Epitope Tag
(2)
Exosome
(1)
Exosomes
(2)
Fc blocker
(1)
FCM
(1)
Flow Cytometry
(2)
Fluoppi
(2)
Fluorescent Proteins
(4)
Gene Regulation
(2)
gfp
(1)
IL-18
(6)
Immune Response
(5)
Immunology
(10)
Inflammation
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isotype control
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keima red
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kinases
(1)
Kits and Assays
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LC3
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Liquid Biopsy
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Metabolism
(4)
MHC
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mitochondria cell death
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mitophagy
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NCP
(3)
Neuroscience
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organoid
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p62
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pathways
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PCSK9
(2)
PCSK9 ELISA Kit
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Peptide
(3)
Peptide Exchange
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phosphorylation
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Preservation
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pro IL-18
(3)
Purify exosome
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Quality Control
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QuickSwitch
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RBP
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Regulation
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rfp
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RiboTrap
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RIP Chip
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RNA
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S100 Proteins
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SARS-CoV-2
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t cell
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TransFix
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