As 2016 draws to a close, let’s look back on some significant advances made in science.
The process of autophagy was first recognized in the late 1950s and it was thought of as bulk “junk” removal. Then, dedicated scientists did a little more digging and found there is an amazing methodology to the “junk” removal. It was discovered that there is rhyme and reason for how a cell decides if and when its components should undergo degradation. Autophagy is rather specific and aids in cell survival by making sure the cell has essential components during times of flux. Autophagy can be selective (i.e. mitophagy) or non-selective (i.e. starvation-induced). Using this method, the cell can have non-essential components recycled or send them to onto autophagolysosomes to be degraded. It is more efficient for a cell to be able to recycle as much as it can, rather than to wait for new proteins to come into the picture. Autophagy is also crucial for clearing out “junk” such as misfolded or aggregated proteins. Research in autophagy has helped provide a better understanding for how a cell is able to survive even under poor conditions.
What is mitophagy? It is when damaged mitochondria are removed from the cell by autophagy. The damaged mitochondria end up in lysosomes for their final disposal. This whole process is to maintain and assure proper cellular function. The importance of this biological process is that it has been implicated in disease states such as cancer and Parkinson’s disease.
When you start working in a new lab or working on a new research project, one of the first things to do is look up publications and learn about the field and what has been accomplished. This will help determine the experiments you will run. It gives you a foundation to build on. But how do you read a publication? How do you know it’s worth your while to muster through? Some publications are miles long with a million figures! EEKS! Don’t be frightened. Keep reading to find out how to tackle a complicated publication and not go crazy.
You have an RNA of interest aka your favorite RNA. The question is: which RBPs interact with it? Who are these RBPs and what are their intentions with your precious RNA?
What happens when you use a black well plate for a fluorometric ELISA assay reading? What happens when you use a clear well plate for a colorimetric ELISA assay reading? Well the short answer is that you made the right plate decision!
Topics: Kits and Assays
What does Autophagy Flux even mean? It’s not in the Urban dictionary. Dear reader, you have come to the right place to learn a little more about autophagy flux and how our Autophagy Watch kit can help you.
You have been given the mission to start a new project. A tiny tube is put in your hands and you’re told it contains a potentially interesting protein and your job is to characterize it. After you wipe nervous sweat from your brow, you get down to business. Step one- how to isolate your new buddy? Immunoprecipitation can help with this question! You determine which epitope tag is on your protein and go to mblintl.com to see what you can buy to help this new adventure. You see there are antibodies conjugated to magnetic beads, which help protein pull downs from cell lysates. It's easy, it's fast. Hmmmm- promising.
Topics: Epitope Tag
What are LC3 isoforms all about? What do they do? How are they different? How are they involved in autophagy?
Antibodies are wonderful. They can act as little detectives to help you determine if the protein you’re interested in is involved in a certain process or help characterize the function. But what if your favorite protein has two forms? Is your antibody sophisticated enough to be able to detect one form over the other? Does this knowledge help you? How? IL-18 is one such protein that has two forms. It is present when it is inactive and present when it is active. So what is known about IL-18 and its function and the two forms it can exist in?