---
title: Tips for successful results using our extremely popular IL-18 ELISA kits
description: Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor requiring processing by caspase-1 into an active cytokine.
image: https://blog.mblintl.com/hubfs/IL18blog.png
---

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- Tips for successful results using our extremely popular IL-18 ELISA kits

# Tips for successful results using our extremely popular IL-18 ELISA kits

### Topics

- Select Topic - 3D Cell Culture Advancements in Metabolism Research Allergy Antibodies Antibody Antibody Reagents Antibody-Peptide Conjugates Apoptosis Assay and Kits Autoimmune Disease Autophagy BRIC Benefits of Tetramer Assays Biomarker Cancer Cell Biology Cell Culture Reagents Cell Stabilization Custom Peptide Synthesis Cytokine Dextramer Staining Drug Discovery ELISA Epigenetics Epitope Tag Exosome Exosomes FCM Fc blocker Flow Cytometry Flow Cytometry Antibodies Fluoppi Fluorescent Proteins Future of Tetramer Technology Gastric Inhibitory Peptide Gene Regulation Gene Therapy Green Fluorescent Protein IL-18 Immune Response Immunology Inflammation Isotype Control Kits and Assays LC3 Liquid Biopsy MHC MHC Alleles MHC Class II Tetramers MHC Peptides Contribute MHC Tetramer MHC Tetramer Protocol MHC tetramer advancements Metabolism Mitophagy NCP Neuroscience Neuroscience Reagents Organoid PCSK9 PCSK9 ELISA Kit Peptide Peptide Exchange Peptide Synthesis Methods Peptide Synthesis Service Peptide-Infused Skin Care Products Preservation Purify exosome Quality Control QuickSwitch Quickswitch™ Custom Tetramer Kits RBP RIP Chip RNA Recombinant Protein Production Recombinant Proteins Regulation RiboTrap S100 Proteins SARS-CoV-2 T-Cell Detection TCR Tetramers Tetramer Tetramer Analysis Tetramer Assay Tetramer Assay Protocol Tetramer Staining TransFix Vectors Western Reagents atg8 covid-19 gfp keima red kinases mitochondria cell death p62 pathways phosphorylation pro IL-18 reagents rfp t cell tuberculosis ultracentrifugation vaccine

### Top Posts

### Subscribe to our Blogs

Published by [Tomohiro Nakajo](https://blog.mblintl.com/author/tomohiro-nakajo) on May 30, 2018 1:00:00 PM

![](https://blog.mblintl.com/hubfs/IL18blog.png)

Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor requiring processing by caspase-1 into an active cytokine. IL-18 plays a major role in the production of interferon-γ (IFNγ) from T cells and natural killer cells. Refresh your memory by reviewing a couple of [previous blog posts.](https://blog.mblintl.com/topic/il-18)

MBL is the first supplier of antibodies and ELISA kits for IL-18. These reagents have become the gold standard worldwide in IL-18 research area. You may have heard about clone #125-2H or ELISA kit code [#7620](https://products.mblintl.com/products/7620) since they are developed and manufactured by MBL.

Our ELISA kit for human IL-18 (code [#**7620**](https://products.mblintl.com/products/7620)) and mouse IL-18 (code [#**7625**](https://products.mblintl.com/products/7625)) are the most cited kits for IL-18. Therefore, we have been able to accumulate significant information from researchers. For example, researchers have shown our ELISA kits can be used to detect IL-18 levels in various samples that we have not tested. Our ELISA kits have been tested using serum, plasma, and cell culture supernatant by MBL. However, as publications are released, we have learned that it also can be used for urine, tissue homogenate, etc.  If you want to use other samples to measure IL-18, please contact us. We can lead you to the correct citation to help you.

- Human urine samples 
    - Am Soc Nephrol. 2010 Jan; 21(1):189-97. (PMID: 19762491)
    - Nephrol Dial Transplant. 2008 Feb; 23(2):566-72. (PMID:17911094)
    - J Am Soc Nephrol. 2005 Oct; 16(10):3046-52. (PMID:16148039)

- Mouse tissue homogenate 
    - J Biol Chem. 2012 Nov 23; 287(48):40391-9. (PMID: 23027874)
    - J Biol Chem. 2012 May 11; 287(20):16955-64. (PMID: 22461621)
    - Int Immunol. 2010 Jul; 22(7):561-70. (PMID: 20497957)

Another frequent inquiry pertains to “cross reactivity to pro-IL-18”.  We have answers for this question! We measured the OD values of recombinant pro-IL-18 when we constructed ELISAs. As a result, human pro-IL-18 OD was around 0.7% OD of human mature IL-18 standard. In mouse, it was less than 10%. Therefore, we can answer that the cross-reactivity of [#7620](https://products.mblintl.com/products/7620) with pro-IL-18 is around 0.7%, and [#7625](https://products.mblintl.com/products/7625) is less than 10%.

Please do not hesitate to contact us if you have any questions. We are happy to help you based on knowledge we have obtained by offering gold standards for detecting IL-18.

 

Tags: [IL-18](https://blog.mblintl.com/topic/il-18), [ELISA](https://blog.mblintl.com/topic/elisa), [pro IL-18](https://blog.mblintl.com/topic/pro-il-18)

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