MBLI is proud to continuously provide high quality research reagents to the biological community, which has led to numerous publications and scientific advances throughout the year. Please contact us if you do not see your specific product of interest.
This newsletter highlights some of the 2022 publications for select research topics.
Content of Newsletter
1. Organoid culture
1. ORGANOID CULTURE
Organoid culture is a 3-dimentional culture method that enables the recapitulation of the tumor microenvironment and the success rate reported is higher than the 2-dimentional culture.
Organoid Culture Product Highlight
Autophagy is a cellular self-digestion process for the purpose of providing nutrients to allow for cell survival during stress conditions. Autophagy can be selective (mitophagy) or non-selective (nutrient poor). Autophagy plays an important role in maintaining the cell homeostasis by removing non-essential cell components. The role of autophagy is generally understood to be securing nutrient sources through autodigestion to survive starvation conditions. However, in recent years, it has been found to work along with the proteasome system in the metabolism of cell components even in normal environments. Compared to proteasomes, which selectively degrade ubiquitinated proteins as their targets, in autophagy the space within the cell is entirely digested; therefore, it is called a bulk degradation system. Recent research and advances have shown an association of mammalian autophagy with diseases such as neurodegenerative disease, infection disease, cardiac disease as well as cancers.
Autophagy Product Highlights
1. PM045: Anti-p62 (SQSTM1) (Human) pAb (Polyclonal Antibody)
p62/SQSTM1 interacts with various molecular groups such as RIP, TRAF6, ERK, aPKCs, and poly-ubiquitin through PB1 domain, Zn finger domain, and UBA domain. This protein directly interacts with LC3, which is localized on autophagosome membrane, and is degraded by autophagic-lysosome pathway. p62 regulates ubiquitin-positive protein aggregates caused by autophagy deficiency.
This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa).
Total p62 ELISA Kit is used for the quantitative measurement of human or mouse p62 protein in cell lysate.
Mitophagy is the selective degradation of old or depolarized mitochondria by autophagy and contributes to maintaining a healthy population of mitochondria. Since damaged mitochondria lead to collapse cell homoeostasis, mitophagy is believed to be protective against diseases related to mitochondrial dysfunction such as in neurodegenerative disorders.
Mitophagy Product Highlight:
Mitophagy detection vectors are designed for the co-expression of MT-mKeima-Red. (CoralHue®Mitochondria-targeted monomeric Keima-Red) gene and mouse Parkin (mPark2) gene from the internal ribosome entry site (IRES), as part of a bicistronic transcript in mammalian cells. Keima-Red has been cloned from Montipara sp. A monomeric version of CoralHue®Keima-Red (mKeima-Red) displays a bimodal excitation spectrum with peaks at 440 and 586nm in neutral and acidic solutions, respectively, and an emission maximum at 620nm. Mitochondrial targeting of mKeima-Red is achieved by introducing a mitochondrial signal sequence at the N-terminus of mKeima-Red.
Apoptosis, a form of programmed cell death, is not only a major process within normal homeostasis but is present in disease states such as cancer and many autoimmune and neurodegenerative diseases. Apoptosis is accompanied by condensation of cytoplasm, loss of plasma membrane microvilli, condensation and fragmentation of nuclei, and extensive degradation of chromosomal DNA into oligomers of about 180 bp. A large number of molecules are involved in carrying out and regulating apoptosis.
Apoptosis Product Highlight:
The MEBCYTO® Apoptosis Kit (Annexin V-FITC Kit) (4700) can identify cells in an earlier stage of apoptosis.
The epigenetics field continues to gain significance as we discover there are more causes to DNA mutations outside of hereditary contribution. Gene expression that is turned on/ off due to external factors, such as environment or diet, continue to draw researchers to discover exactly how these genes become altered.
RIP-Assay Kit is optimized for performing the RIP-Chip process. In the RIP-Assay protocol, mRNP complexes are isolated from cell extracts by immunoprecipitation with RIP-Certified Anti-RBP Antibodies provided from MBL. mRNAs are isolated from mRNPs using guanidine hydrochloride. Thus, RIP-Assay Kit does not contain phenol or chloroform, allowing safe isolation of “high-quality RNA” from RNP complexes without degradation. Once purified, the RNAs present in the complex are analyzed to identify the target mRNAs using various molecular biology tools such as RT-PCR, gene expression analysis based on microarray technology (Chip analysis), or sequencing.
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