The Lab Notebook

Improve your neutralization using monoclonal IL-18 antibodies

Posted by Tomohiro Nakajo on Jun 19, 2018 1:00:00 PM

Interleukin-18 (IL-18) is a member of the IL-1 superfamily and synthesized as an inactive precursor that requires processing by caspase-1 to become an active cytokine. IL-18 plays a major role in the production of interferon-γ (IFN-γ) from T cells and natural killer cells. Refresh your memory by reviewing a couple of previous blog posts.

MBL offers various human, mouse and rat antibodies for IL-18 that can be used for multiple applications.

Code# Product Name Clone Isotype Size Applications
D044-3M2 Anti-IL-18 (Human) mAb (Functional Grade) 125-2H Mouse IgG1κ 100 μg/100 μL IP, NT
D048-3M2 Anti-IL-18 (Human) mAb (Functional Grade) 93-10C Rat IgG1 100 μg/100 μL IP, NT
D043-3 Anti-IL-18 (Human) mAb 25-2G Mouse IgG1κ 100 μg/100 μL WB, IH*
D044-3 Anti-IL-18 (Human) mAb 125-2H Mouse IgG1κ 100 μg/100 μL IP, ELISA, NT, IC*
D045-3 Anti-IL-18 (Human) mAb 159-12B Rat IgG2a 100 μg/100 μL IP
D045-6 Anti-IL-18 (Human) mAb-Biotin 159-12B Rat IgG2a 25 μg/50 μL ELISA
PM014 Anti-IL-18 (Human) pAb Polyclonal Rabbit IgG 100 μL WB, IH
M156-3 Anti-pro-IL-18 (Human) mAb 43A11 Mouse IgG1 κ 100 μg/100 μL WB
D046-3 Anti-pro-IL-18 (Human) mAb 39-3F Rat IgG2a 100 μg/100 μL WB, Function
D047-3 Anti-pro-IL-18 (Human) mAb 74 Rat IgG2a 100 μg/100 μL WB*, IP
D048-3 Anti-pro-IL-18 (Human) mAb 93-10C Rat IgG1 100 μg/100 μL IP, NT
D048-6 Anti-pro-IL-18 (Human) mAb 93-10C Rat IgG1 50 μL ELISA
M157-3 Anti-IL-18 (Rat) mAb 21A12 Mouse IgG2b κ 100 μg/100 μL WB
M158-3 Anti-IL-18 (Rat) mAb 91D8 Mouse IgG2b κ 100 μg/100 μL IP

 

In this blog, we would like to introduce a neutralization method using our IL-18 antibodies. There are many citations where D044-3 (for human) and D048-3 (for mouse) antibodies are used for neutralization studies.  As a result, we have received many requests for bulk orders without glycerol for these particular antibodies. In order to meet researchers needs, we have produced anti-IL-18 functional grade (D044-3M2 and D048-3M2) monoclonal antibodies. Accordingly there is no need for a special order or waiting for antibody preparation. You can order functional grade IL-18 mAb and start neutralization experiments quickly! These antibodies are supplied in neutral solutions without any preservatives, and the endotoxin levels are lower than 0.5/EU mg antibody, as determined by the LAL assay in our QC tests. You can add it into cell culture without worry.

When you add the anti-IL-18 monoclonal antibody into the solution containing IL-18 protein, it will neutralize the activity of IL-18 protein, resulting in suppressed production of IFN-γ in KG-1 (acute myeloid leukemia) cells. This method has been confirmed in our QC tests.

IL18blog1

Moreover, the following citations for neutralization use MBL antibodies.

  • Anti-IL-18 (Human) mAb (D044-3)
    • Nussbaumer O et al., Blood. 118, 2743-51 (2011) (PMID: 21673342)
    • Tu Z et al., J Exp Med. 205, 233-44 (2008) (PMID: 18195076)
    • Sugawara S et al., J Immunol. 167, 6568-75 (2001) (PMID: 11714826)
  • Anti-IL-18 (Mouse) mAb (D048-3)
    • Kahlenberg JM et al., J Immunol. 190, 1217-26 (2013) (PMID: 23267025)
    • Matsunaga H et al., Am J Physiol Gastrointest Liver Physiol. 301, G555-64 (2011) (PMID: 21719737)
    • Humann J, Lenz LL., J Immunol. 184, 5172-8 (2010) (PMID: 20351186)
    • Chaix J et al., J Immunol. 181, 1627-31 (2008) (PMID: 18641298)
    • Beadling C and Slifka MK., Blood 105, 1179-86 (2005) (PMID: 15471952)
    • Wang SZ et al., J Immunol. 173, 4040-9 (2004) (PMID: 15356153)
    • Malmgaard L and Paludan SR., J Gen Virol. 84, 2497-500 (2003) (PMID: 12917471)
    • Fukao T et al., J Immunol. 164, 64-71 (2000) (PMID: 10604994)

If you are interested in IL-18 neutralization, you can start quickly and easily by using our products and these citations. 

 Highly cited human and mouse IL-18 ELISA kits

Tags: IL-18, ELISA, pro IL-18

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