The Lab Notebook

Antibodies for fluorescent proteins: Will it cross react with my variant?

Posted by Tomohiro Nakajo on Feb 27, 2018 1:00:00 PM

Green fluorescent protein (GFP) was first isolated by Dr. Osamu Shimomura in 1961. Since then, fluorescent protein (FP) technology has made drastic advancements by many researchers. Currently, FP technology is the most popular tool for visualizing target proteins. Dr. Shimomura, Martin Chalfie and Roger Y. Tsien won the 2008 Nobel Prize in Chemistry to mark their great achievements in life science research.

FluorescentBlog.jpgMBLI offers the CoralHue® FP series such as Keima-Red, Kusabira-Orange, and many others. We also offer antibodies agaist FPs.

FP antibodies are used for multiple purposes including pull-down assays, used as epitope-tag, increasing fluorescent intensity if the FP fluorescence is not bright enough, as well as other applications. MBLI offers polyclonal and monoclonal anti-GFP and anti-RFP antibodies with various conjugates including HRP, Agarose, and Magnetic beads.  In fact, many researchers prefer to use our bead conjugates for pull-down assays. Our GFP polyclonal antibody (598) is highly regarded to have high performance and reliability among many reseachers. There are over 500 citations to date using this FP antibody.

Code# Description Clone Applications Conjugates Available Notes
598 Anti-GFP pAb Polyclonal WB, IP, IC, IH, ChIP*, Immunoelectron Microscopy HRP Cumulative citations over 500
D153-3 Anti-GFP mAb RQ2 IP, IC Agaraose, Magnetic beads, Magnetic Agarose, Alexa-Fluor Excellent performance in IP and staining
PM005  Anti-RFP pAb Polyclonal WB, IC, IH* HRP Highly cited
M165-3  Anti-RFP mAb 3G5 IP, FCM, IC Agarose, Magentic beads, Magentic Agarose Specialized for IP
M204-3  Anti-RFP mAb 1G9 WB HRP Specialized for WB
M208-3 Anti-RFP mAb Cocktail (1G9 and 3G5) WB, IP, FCM, IC   For multiple applications

 

There are many FP variants available today from different resources. Are there the same number of antibodies as FP variants? The answer is no, because FP antibodies have the possibility to cross react to various variants due to high homology among those variants. Our antibodies have been tested for cross reactivity to some variants as shown in the *table below. An open circle shows we have tested our FP antibody with the corresponding variant.  N.T. indicates that we have not tested against the variant.

Cross Reactivity GFP_resized.jpg *Reported in articlesFluorescentBlog3.jpg*Reported in articles

Please don’t fret if you don’t see your variant listed!  If your FP is not seen in the table above, we can estimate cross reactivity by running a homology search between our antigen and your FP. The following link is a protein BLAST search between our GFP antigen (full length of GFP derived from Aequorea victoria) and your FP. If the homology is high enough, polyclonal antibody (598) has a good possibility that it will cross react with your FP because this antibody has multiple epitopes.

Run Blast

And of course- if you have any questions, please don't hesitate to contact us!

Tags: Fluorescent Proteins, Antibodies