--- title: Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform description: Evaluate the binding affinity of interesting peptides with the HLA-A*02:01 allele using QuickSwitch kits. image: https://blog.mblintl.com/hubfs/quickswitch%20quant%20assay.jpg --- **Please Note:** MBL International will be shutting down its operations effective December 31, 2024. Distribution of MBL products in the United States will be transferred to Cosmo Bio US on January 1st while European Distributors will remain unchanged. For any US inquiries regarding orders or support during this transition, reach out to Cosmo Bio: [https://www.cosmobiousa.com/](https://www.cosmobiousa.com/). For Non-US inquiries reach out to MBL in Japan: [https://www.mblbio.com/](https://www.mblbio.com/). 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[Home](https://blog.mblintl.com/) - [Blogs](https://blog.mblintl.com) - Evaluating the biological activity of a cancer vaccine with the HLA-A\*02:01 QuickSwitch™ Quant platform # Evaluating the biological activity of a cancer vaccine with the HLA-A\*02:01 QuickSwitch™ Quant platform ### Topics - Select Topic - 3D Cell Culture Advancements in Metabolism Research Allergy Antibodies Antibody Antibody Reagents Antibody-Peptide Conjugates Apoptosis Assay and Kits Autoimmune Disease Autophagy BRIC Benefits of Tetramer Assays Biomarker Cancer Cell Biology Cell Culture Reagents Cell Stabilization Custom Peptide Synthesis Cytokine Dextramer Staining Drug Discovery ELISA Epigenetics Epitope Tag Exosome Exosomes FCM Fc blocker Flow Cytometry Flow Cytometry Antibodies Fluoppi Fluorescent Proteins Future of Tetramer Technology Gastric Inhibitory Peptide Gene Regulation Gene Therapy Green Fluorescent Protein IL-18 Immune Response Immunology Inflammation Isotype Control Kits and Assays LC3 Liquid Biopsy MHC MHC Alleles MHC Class II Tetramers MHC Peptides Contribute MHC Tetramer MHC Tetramer Protocol MHC tetramer advancements Metabolism Mitophagy NCP Neuroscience Neuroscience Reagents Organoid PCSK9 PCSK9 ELISA Kit Peptide Peptide Exchange Peptide Synthesis Methods Peptide Synthesis Service Peptide-Infused Skin Care Products Preservation Purify exosome Quality Control QuickSwitch Quickswitch™ Custom Tetramer Kits RBP RIP Chip RNA Recombinant Protein Production Recombinant Proteins Regulation RiboTrap S100 Proteins SARS-CoV-2 T-Cell Detection TCR Tetramers Tetramer Tetramer Analysis Tetramer Assay Tetramer Assay Protocol Tetramer Staining TransFix Vectors Western Reagents atg8 covid-19 gfp keima red kinases mitochondria cell death p62 pathways phosphorylation pro IL-18 reagents rfp t cell tuberculosis ultracentrifugation vaccine ### Top Posts ### Subscribe to our Blogs Published by [Lise Peeters, PhD](https://blog.mblintl.com/author/lise-peeters-phd) on Dec 11, 2017 11:33:12 AM ![](https://blog.mblintl.com/hubfs/quickswitch%20quant%20assay.jpg) Immuno-oncology is a growing field in which different strategies such as immune modulators, vaccines and adoptive cell therapies are applied for new immunotherapies. Cancer vaccine immunogenicity and stability are two important parameters in vaccine development. In addition, vaccine immunogenicity implies accurate enumeration of antigen specific T-cells. MHC tetramers can help monitor peptide vaccine immunogenicity and stability. T-cell receptors (TCR) recognize a unique MHC-peptide combination. The half-life of the MHC-Peptide complex on the cell surface is proportional to the peptide affinity with the MHC molecule. Peptide exchange occurs in vivo during biosynthesis of MHC class I molecules. The QuickSwitch**™** principle is based on this system. The QuickSwitch**™** platform allows determining peptide binding to MHC molecules. Moreover, the percentage of peptide exchange can be calculated. Evaluating the biological activity of a cancer vaccine is thus an important application of this kit. To illustrate this application the DepoVax**™**–based ovarian cancer vaccine candidate, DPX-Survivac, is used. The vaccine consists of a mixture of liposomes and different peptides, covering different MHC alleles. Peptides, encapsulated by liposomes, are recognized by antigen presenting cells (APC) and are transferred to the cell surface by MHC molecules. T-lymphocytes bind to the APC cells in an MHC/peptide dependent manner and are activated, proliferate, and subsequently kill tumor cells that display the same MHC/peptide complexes as the APCs. ## Principle of the cell-based T2 shift assay and the cell-free QuickSwitch**™** Quant assay ### T2 shift assay T2 cells are a human cell line that express low levels of HLA-A2 on their surface due to deficiency in TAP protein. Upon incubation with a peptide that can bind to HLA-A2, expression of HLA-A2 on the surface increases as the peptide stabilizes the complex.  After antigen stimulation, the relative HLA-A2 expression in vitro is quantified by immunofluorescent staining of T2 cells with a fluorescein-conjugated monoclonal antibody (anti-HLA-A2). ### QuickSwitch™ Quant Tetramer assay The kit includes a tetramer with an irrelevant, low affinity binding exchangeable peptide, a peptide exchange factor, HLA capture beads and a FITC conjugated antibody, which recognizes the exiting peptide. ![quickswitch quant assay.jpg](https://blog.mblintl.com/hubfs/quickswitch%20quant%20assay.jpg) Figure 1:  The  FITC conjugated antibody  recognizes the exiting peptide. When the exiting peptide is not displaced, a high mean fluorescent intensity (MFI) can be measured via the flow cytometer. On the other hand, a competing peptide that binds to the HLA complex will displace the exiting peptide proportionally to its affinity, resulting in a lower MFI. In this study the SurA2.M peptide with the following sequence, LMLGEFLKL, is used. The predicted (IEDB artificial neural network method) HLA-A2 affinity of the peptide is 25,41 nM. Do you want to know more about the materials and methods, have a look at our [AAI QuickSwitch™ poster](https://blog.mblintl.com/hubfs/wp-content/uploads/45x45-AAI-poster.pdf). ## Comparison of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay ![cell free quickswitch quant assay.jpg](https://blog.mblintl.com/hubfs/cell%20free%20quickswitch%20quant%20assay.jpg) Figure 2: Quantification of SurA2.M peptide binding to HLA-A2 using the T2 HLA-A2 shift assay (A) and the HLA-A2 QuickSwitch™ Quant Tetramer assay (B). We could find that the HLA-A2 QuickSwitch™ Quant peptide exchange assay can be used to evaluate the biological activity of peptide-based vaccines. Similar to the T2 shift assay, QuickSwitch™ quantifies HLA-A2/peptide interactions, but does this in a cell-free environment. Sensitivity of the HLA-A2 QuickSwitch™ Quant is at least 200x higher than the HLA-A2 T2 shift assay. The HLA-A2 QuickSwitch™ Quant peptide exchange assay is user-friendly, fast and can be performed in a high-throughput 96-well plate format.   --- [Back to Blog](https://blog.mblintl.com) --- ### Comments Or Suggestions ## Related Articles ### Sign up for our latest news and promotions. 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