The Lab Notebook

Who’s that on my mRNA?

Posted by Bindi M. Doshi, PhD on May 17, 2016 2:44:01 PM

You have an RNA of interest aka your favorite RNA. The question is: which RBPs interact with it? Who are these RBPs and what are their intentions with your precious RNA?

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Topics: RNA, Epigenetics, Gene Regulation, RiboTrap, RBP

Don’t rip your hair out- use our RIP certified antibodies!

Posted by Bindi M. Doshi, PhD on May 2, 2016 7:47:32 AM
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What are RBPs (RNA binding proteins)? Very briefly, they are proteins that bind to RNA. (You probably figured that out already.) More importantly, they have a role in regulating RNA as part of the ribonucleoprotein complex (RNP). They are involved in interactions that regulate posttranscriptional  gene regulation1. Not only are RBPs important because of their interactions, but also because it was found that mutations in RBPs can lead to disease formation 2, 3, 4. In order to investigate which RBPs bind with RNAs, a technique called RNP immunoprecipitation (RIP) can be used5.  And in order to have an amazing RIP experiment, one needs an equally amazing antibody.
 
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Topics: RIP Chip, RNA, Epigenetics, Gene Regulation