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Blocking Fc receptors for flow cytometry

Posted by Wushouer Ouerkaxi, Ph.D. on Oct 17, 2019 1:00:00 PM

While, in theory, using antibodies against Fc Receptors (FcRs) is the best way to eliminate unwanted signals mediated by FcR binding, you may not always have a well-optimized antibody pool against FcRs sitting right in front of you for your experiment. Furthermore, such a blocker may not be applicable when you have one of the FcRs, such as CD16, CD32 or CD64 to measure in your channel. In this blog, we share an "old school" way of blocking by just using a serum that is routinely available in most labs.

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Topics: Antibodies, Fc blocker

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