The Lab Notebook

Shed a little light in your cells using anti-GFP

Posted by Bindi M. Doshi, PhD on Oct 24, 2018 1:02:00 PM

In 2008, the Nobel Prize in Chemistry was awarded to Dr. Roger Y. Tsien, Dr. Osamu Shimomura, and Dr. Martin Chalfie for their discovery and development of the green fluorescent protein (GFP).  Since then, fluorescent protein (FP) technology has made drastic advancements by many researchers.  FP antibodies are used in multiple applications.

GFP is composed of 238 amino acids and has a molecular weight of 27 kDa.  The beautiful green color is observed when the protein is exposed to blue-ultraviolet light.  Excitation occurs at 488nm and emitted at 509nm.  Being able to have GFP gene expression in the researcher’s choice of cell line has revolutionized and expanded biological research possibilities.  Countless GFP fusion recombinant proteins have been made aiding in characterizing particular proteins of interest.  To be able to quantify protein expression, antibodies to GFP can be used and analyzed using western blot.   Anti-GFP antibodies can also be used for IHC, ICC, IPP, ChIP, and EM analysis, based on the particular antibody and its specifications.

Red fluorescent protein (RFP) is another very popular fluorescent protein that has a molecular weight of 25.9 kDa.  It has excitation at 558nm and emission at 583nm.  RFP expression is sometimes used in combination with GFP expression, however, one must be careful of signal overlap.  As with anti-GFP antibodies, anti-RFP antibodies can be used for various applications, depending on its specifications.

MBLI offers polyclonal and monoclonal anti-GFP and anti-RFP antibodies with various conjugates including HRP, Agarose and Magnetic beads.

598

m15503

Our GFP polyclonal antibody (product code # 598) is known to have high performance and reliability among many researchers. There are over 500 citations to date using this anti-GFP antibody. In addition to this GFP antibody, we also offer many other antibodies with different cross reactivities.

Product Highlights

Product Code Product Name Application
598 Anti-GFP (Green Fluorescent Protein pAb ChIP, EM, ICC, IHC, IP, WB
598-7 Anti-GFP pAb HRP-DirecT  WB
598-7B Anti-GFP HRP DirecT: Trial Size WB
598B GFP Polyclonal Antibody:Trial Size ChIP, EM, ICC, IHC, IP, WB
D153-10 Anti-GFP mAb-Magnetic Agarose IP
D153-11 Anti-GFP (Green Fluorescent Protein) mAb-Magnetic Beads IP
D153-3 Anti-GFP (Green Fluorescent Protein) mAb ICC, IP
D153-3B Green Fluorescent Protein (GFP) (RQ2) Monoclonal Antibody: Trial Size ICC, IP
D153-6 Anti-GFP mAb-Biotin ELISA
D153-8 Anti-GFP (Green Fluorescent Protein)mAb-Agarose IP
D153-A48 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor 488 ICC
D153-A59 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor 594 ICC
D153-A64 Anti-GFP (Green Fluorescent Protein) mAb-Alexa Fluor 647 ICC
M048-3 Anti-GFP (Green Fluorescent Protein) mAb ICC, IHC, IP, WB
PM073 Anti-Renilla GFP pAb FCM, ICC, IP, WB
M155-3 Anti-RFP mAb ICC, WB, IHC
M165-10 Anti-RFP mAb-Magnetic Agarose IP
M165-11 Anti-RFP mAb-Magnetic Beads IP
M165-3 Anti-RFP mAb IP
M165-8 Anti-RFP mAb-Agarose IP
M204-3 Anti-RFP mAb WB
M204-7 Anti-RFP mAb-HRP-DirecT WB
M208-3 Anti-RFP mAb Cocktail FCM, ICC, IP, WB
PM005 Anti-RFP pAb ICC, IHC, WB
PM005-7 Anti-RFP pAb-HRP-DirecT WB


 

Tags: gfp, rfp, Antibodies

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