The Lab Notebook

S100 Proteins: Study their Hundreds of Functions with MBL ELISA Kits

Posted by Tomohiro Nakajo on Jan 24, 2018 1:00:00 PM

Have you heard of the S100 protein family? S100 refers to proteins that are 100% soluble in saturated ammonium sulfate solution. They were first discovered as a major protein fraction (0.6% of total soluble proteins) isolated from bovine brain by B.W. Moore in 1965.

The S100 protein family consists of calcium binding proteins with 2 distinct helix-loop-helix motifs (EF-hands), existing as a homodimer. Currently 20 family members are known and have been found to be secreted extracellularly as well as to be involved in intracellular signaling. Many of the functions of the S100 protein family are currently unknown. Recent research has suggested an association with various diseases such as cancer, inflammation, and atherosclerosis. For example, levels of S100A4, A6, A14, and P are upregulated in many cancers. S100A7 has been studied in skin inflammatory disease and S100A12 and Calprotectin (only one heterodimer consisting of S100A8 and A9) have been studied in inflammatory bowel disease. 

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MBLI offers 12 sandwich ELISA kits for this protein family. If you want to know detailed information of each isoform of S100, please visit our website

MBLI S100 ELISA kits are highly cited, particularly our S100A12 kit. Below is a partial list of citations with sample type and disease correlations. These kits have been used for the analysis of many diseases using various samples, not only serum/plasma but also cerebrospinal fluid (CSF), bronchoalveloar lavage fluid (BALF), and more. A citation highlight includes the measurement of S100A12 (CY-8058V2) in a stool sample. MBLI's S100A7 ELISA kit (CY-8073) can be used with the sample of washing fluid from skin. This sample preparation method is written in its datasheet

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Lastly, I would like to introduce MBLI's new S100 ELISA kit, Calprotectin. As mentioned above, Calprotectin is a heterodimer of S100A8 and A9, and well known as a biomarker for Inflammatory Bowel Disease. Our RUO Calprotectin ELISA kit (CY-8107) can measure the S100A8 and A9 heterodimer, without cross reactivity with each homodimer. We employ capture antibody recognizing both S100A8 homodimer and Calprotectin and a detection antibody recognizing only Calprotectin. MBLI's S100A8 (CY-8061) and S100A9 (CY-8062) ELISA kits do not cross-react with Calprotectin.

Given their relevance to a variety of diseases, S100 likely plays a role in your research pathway of interest. Please visit our website to learn more!

Tags: ELISA, S100 Proteins