The Lab Notebook

Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.

Posted by Tomohiro Nakajo on Jan 9, 2018 10:24:11 AM

Mechanisms of Autophagy

The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold protein that interacts with a variety of molecules involved in toll-like receptor (TLR) signaling, such as TRAF6, ERK, and aPKC. Recent evidence suggests that p62 binds directly to the autophagosome marker LC3 and is then selectively degraded by autophagy.

p62 contains multiple phosphorylation sites. Sequential phosphorylation of these sites regulates biological defense mechanisms such as selective autophagy and the Keap1-Nrf2 system. Therefore, the failure of the p62 pathway is associated with various diseases such as cancer and many neurodegenerative diseases.

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To learn more about the mechanisms of p62, please refer to our whitepaper written by Drs. Masaaki Komatsu and Yoshinobu Ichimura, Niigata University. They are leading researchers in Autophagy,  especially p62 and Keap1-Nrf2 interaction. Dr. Komatsu was recognized as one of the most highly cited researchers in Autophagy in 2014 and 2015 by Clarivate Analytics.

White Paper : 62/SQSTM1: The molecule that links autophagy to the Keap1-Nrf2 system

p62 Detection

How do you detect p62 or phospho-p62 using antibodies? Western blotting? Immunohistochemistry?  Quantification of biomarkers is essential for medical and diagnostic application. Selective autophagy and Keap1-Nrf2 are already being discussed for their use as a diagnostic as well as other medical applications.

MBL International has developed new sandwich ELISA kits that can semi-quantify total p62 and its phosphorylation level at Ser403 and Ser349. Phospho-p62 ELISA kits are the first kits to quantify these markers by ELISA in the world research market. It is a chance to get a new discovery in the Autophagy area!

CY-7055: CycLex® Total p62 ELISA Kit
CY-7057: CycLex® Phospho-p62 Ser403 ELISA Kit
CY-7056:  CycLex® Phospho-p62 Ser349 ELISA Kit

These kits can help you to prepare cell lysate easily including cell lysis buffers. Phosphatase inhibitor solution is also included in the phosho-p62 kits. They can be used for human and mouse samples.

Total p62 and phospho-p62 levels in HeLa cells

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We also offer antibodies against p62 and phopho-p62. MBLI offers highly cited polyclonal and monoclonal p62 antibodies that can be used for multiple applications. Fluorescent dyes or HRP conjugated antibodies are also offered. Discover your perfect one from the following list.

Code # Product Name Clone Host Species Cross-reactivity Application Conjugate
M162-3 Anti-p62 (SQSTM1) (Human) mAb 5F2 Mouse Hu WB, IP, IF, IHC, FCM  
M162-A48 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 488 5F2 Mouse Hu IF, FCM Alexa Fluor® 488
M162-A59 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 594 5F2 Mouse Hu IF Alexa Fluor® 594
M162-A64 Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 647 5F2 Mouse Hu IF, FCM Alexa Fluor® 647
PM045 Anti-p62 (SQSTM1) (Human) mAb Polyclonal Rabbit Hu, Mo, Rat,Hm WB, IP, IF, IHC  
PM066 Anti-p62 C-terminal (Human) pAb Polyclonal Guinea Pig Hu, Mo, Rat, Hm WB, IP, IF, IHC  
PM066-7 Anti-p62 C-terminal pAb-HRP-DirecT Polyclonal Guinea Pig Hu, Mo, Rat, Hm WB HRP
M217-3 Anti-Phospho-p62 (SQSTM1) (Ser351) pAb 5D5 Mouse Hu,Mo WB, IF, IHC  
PM074 Anti-Phospho-p62 (SQSTM1) (Ser351) pAb Polyclonal Rabbit Hu,Mo WB, IP, IF, IHC  
D343-4 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb 4F6 Rat Hu,Mo WB,IHC  
D344-3 Anti-Phospho-p62 (SQSTM1) (Ser403) mAb 4C8 Rat Hu,Mo WB,IHC  

Tags: Autophagy, p62