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M. Mamunur Rahman, PhD

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MuLV gp70 (AH1) Tetramer - a great tool for observing antigen specific immune response in cancer

Posted by M. Mamunur Rahman, PhD on Jun 25, 2020 12:00:00 PM

MuLV gp 70, also designated as AH1 (SPSYVYHQF), is an endogenous viral envelope glycoprotein encoded in the mouse genome that is transcriptionally silent in normal tissues. The expression of gp70 is reactivated in a few cancer cell lines including CT26 colorectal cancer and B16 melanoma cells. Other investigators have demonstrated the presence of MuLV-encoded molecules in murine lymphomas and leukemias as well as Simian Virus 40 (SV40)-transformed NIH-3T3 cells. Researchers measure total T cell and T cell subset by detection of various cell surface molecules to gain better insight into the central role of the immune system. MBL International offers MHC tetramer reagents for murine model antigens, tumor antigens, viral antigens, etc. MHC-peptide tetramer complexes detect antigen specific T-cells at the single cell level, allowing for accurate measurements of the functional immune response in cancer, infectious diseases, and automimmune diseases.

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Topics: Tetramer

Is Wnt3a an essential component in organoid culture?

Posted by M. Mamunur Rahman, PhD on Mar 27, 2019 2:00:00 PM

Organoids are self-organized three-dimensional tissue cultures that are derived from mammalian pluripotent or adult stem cells. These in-vitro-grown cell clusters are given possibilities to replicate much of the complexity of an organ. There are potentially as many types of organoids as there are different tissues and organs. To date, researchers have been able to prepare brain, kidney, lung, intestine, stomach, liver and many more are developing.

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Topics: 3D Cell Culture, NCP, organoid

Can NanoCulture Plate (NCP) be a better 3D cell culture platform?

Posted by M. Mamunur Rahman, PhD on Mar 28, 2018 1:01:00 PM

Cell-based assays are the first step for drug screening applications and have been performed in monolayer culture condition for decades. These traditional cell cultures have been known to enhance integrin signals, which mask several biologic activities in tumor cells. In contrast, it is already known that 3D cell culture can replicate the intra-tumor microenvironment.

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Topics: 3D Cell Culture, NCP

Why NCP 3D cell culture method helps your experiments become more real

Posted by M. Mamunur Rahman, PhD on May 18, 2017 10:04:00 AM

3D cell culture innovation ensures that time spent at the bench will correlate to in vivo environments, with possibilities to aid in future discoveries such as drug delivery into tumor cells or understanding tissue microenvironment. Typically, a researcher use a conventional 2D cell culture technique but are limited to what observations they can truly make. NanoCulture Plate 3D platform has been designed to facilitate researchers’ time in the lab.

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Topics: Cell Biology, 3D Cell Culture, NCP

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