Organoids are self-organized three-dimensional tissue cultures that are derived from mammalian pluripotent or adult stem cells. These in-vitro-grown cell clusters are given possibilities to replicate much of the complexity of an organ. There are potentially as many types of organoids as there are different tissues and organs. To date, researchers have been able to prepare brain, kidney, lung, intestine, stomach, liver and many more are developing.
There is an increase in demand for scientists to show cell migration and proliferation on a surface that more closely mimics an in vivo environment. Using a gel surface can be tricky. Therefore, MBL has a unique and innovative technology where a proprietary plastic film is used for plating cells. This technology is easy to use with many different cell types and is reproducible every time. Please see the table below comparing various techniques for 3D cell cultures:
Cell-based assays are the first step for drug screening applications and have been performed in monolayer culture condition for decades. These traditional cell cultures have been known to enhance integrin signals, which mask several biologic activities in tumor cells. In contrast, it is already known that 3D cell culture can replicate the intra-tumor microenvironment.
3D cell culture innovation ensures that time spent at the bench will correlate to in vivo environments, with possibilities to aid in future discoveries such as drug delivery into tumor cells or understanding tissue microenvironment. Typically, a researcher use a conventional 2D cell culture technique but are limited to what observations they can truly make. NanoCulture Plate 3D platform has been designed to facilitate researchers’ time in the lab.