The Lab Notebook

LC3: The gold standard for autophagy research

Posted by Lise Peeters, PhD on Dec 13, 2018 1:00:00 PM

LC3, a mammalian homologue of yeast ATG8, is a soluble protein that is distributed ubiquitously in mammalian tissues and cultured cells. LC3 has been studied most extensively and is frequently used as an autophagy marker in mammals.  Newly translated LC3 (proLC3) is immediately processed at the C-terminus in order to form LC3-I. During autophagy, autophagosomes engulf cytoplasmic components, including cytosolic proteins and organelles. Concomitantly, a cytosolic form of LC3 (LC3-I) is conjugated to phosphatidylethanolamine to form LC3-phosphatidylethanolamine conjugate (LC3-II), which is recruited to autophagosomal membranes.

LC3-1

Autophagosomes fuse with lysosomes to form autolysosomes, and intra-autophagosomal components are degraded by lysosomal hydrolases. At the same time, LC3-II in autolysosomal lumen is degraded. Thus, lysosomal turnover of the autophagosomal marker LC3-II reflects starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death.

Recent studies have demonstrated that mammalian autophagy is involved not only in the starvation response, but also in antigen presentation, cell death, development, aging, tumorigenesis, and in the defense against bacterial infection. Thus, autophagy research will be increasingly important in understanding these processes in the body. In order to determine the levels of autophagy, anti-LC3 antibodies can be used for detection of endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence.

Product Highlights

Product Code Product Name Species Reactivity Applications
M152-3 Anti-LC3 Monoclonal Antibody Human, Mouse, Rat, Hamster FCM, ICC, IP, WB
M186-3 Anti-LC3  Monoclonal Antibody Human, Mouse, Rat, Hamster WB
M186-7 Anti-LC3 Monoclonal Antibody HRP-DirecT Human, Mouse, Rat, Hamster WB
PD014 Anti-LC3 Polyclonal Antibody Human, Mouse, Rat, Hamster WB
PM036 Anti-LC3 Polyclonal Antibody Human, Mouse, Rat, Hamster FCM, ICC, IHC, IP, WB

 

 

Tags: Autophagy, LC3, atg8

Subscribe to Email Updates

Recent Posts